Simultaneous evaluation of plasma membrane integrity, acrosomal integrity, and mitochondrial membrane potential in bovine spermatozoa by flow cytometry
نویسندگان
چکیده
منابع مشابه
Evaluation of Sperm Viability and Acrosomal Integrity by Flow Cytometry Analysis in Jersey Crossbred Bulls
1 Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-7, Tamil Nadu, India 2 Education Cell, Madras Veterinary College, TANUVAS, Chennai-7, Tamil Nadu, India 3 Translational Research Platform for Veterinary Biologicals, Chennai – 51, Tamil Nadu, India 4 Madras Veterinary College, TANUVAS, Chennai-7, Tamil Nadu, India 5 Cen...
متن کاملIntegrity of the plasma membrane, the acrosomal membrane, and the mitochondrial membrane potential of sperm in Nelore bulls from puberty to sexual maturity
This study evaluated the plasma membrane integrity, acrosomal membrane integrity, and mitochondrial membrane potential of Nelore bull sperm from early puberty to early sexual maturity and their associations with sperm motility and vigor, the mass motility of the spermatozoa (wave motion), scrotal circumference, and testosterone. Sixty Nelore bulls aged 18 to 19 months were divided into four lot...
متن کاملDetermination of membrane integrity of canine spermatozoa
Semen samples from 9 sexually mature dogs of different breeds, aged from 1 to 5 years were used. Quality of sperm was evaluated using the following parameters: progressive motility (subjective), then structural and functional integrity of spermal membrane (supravital colouring (eosine/nigrosine) and HOS test). The majority of HOS reactive or swollen spermatozoa was placed in a hypoosmolar solut...
متن کاملToxicity of glycerol for the stallion spermatozoa: effects on membrane integrity and cytoskeleton, lipid peroxidation and mitochondrial membrane potential.
Glycerol is, to date, the most widely used cryoprotectant to freeze stallion spermatozoa at concentrations between 2% and 5%. Cryoprotectant toxicity has been claimed to be the single most limiting factor for the success of cryopreservation. In order to evaluate the toxic effects of the concentrations of glycerol used in practice, stallion spermatozoa were incubated in Biggers Whitten and Whitt...
متن کاملP-73: Effect of Sodium Dodecyl Sulfate and Different Levels of Clove Bud Extract on Motility, Viability and Plasma Membrane Integrity of Ram Spermatozoa during Cooling and Cryopreservation
Background: Hen egg yolk is a common component of most semen cryopreservation extenders. However, diluents containing the egg yolk may have adverse effects on the quality of the semen. Egg yolk could be solubilized by the addition of sodium dodecyl sulphate (SDS) in semen extenders. Many studies have shown beneficial effects of antioxidants addition to semen freezing extenders. Antioxidants are...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Zygote
سال: 2015
ISSN: 0967-1994,1469-8730
DOI: 10.1017/s0967199415000490